portable pcr
title_Ultra-Fast_Real-Time_PCR_System
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Sample to Result within 40 min
Ultra-Fast & Highly Sensitive
Direct Prep without DNA Extraction
Simple to Use & Fully Portable

ASF_Fast_POCT_System

Total Solution for On-Site Diagnosis of ASF
ASF Fast POCT Kit
Palm PCR™ ASFV Direct Prep Kit for ultra-fast ≤ 15 min direct preparation of ASFV DNA from plasma and serum samples of suspected pigs
Palm PCR™ ASFV Direct Prep Kit for ultra-fast ≤ 25 min PCR and real-time detection
Fully-Mobile Equipment
Palm PCR™ S1/S1e Ultra-fast Mobile Real-time PCR System for ultra-fast ≤ 25 min hot-start real-time PCR using Palm PCR™ ASFV Fast PCR Kit
Ahram™ RX1 Portable Centrifuge for on-site preparation of blood samples and real-time PCR mix



Simple, Convenient & Fast Workflow
Step 1. ASFV Direct Prep
1. Add 20 μL of a plasma or serum sample into an ASFV Direct Prep tube.
2. Perform 2-step isothermal incubation on Palm PCR™ S1/S1e device.
3. Centrifuge the incubated Direct Prep tube for 2 min at 8,500 RPM on Ahram™ RX1 Portable Centrifuge, and use 5 μL of the clear
supernatant for real-time PCR diagnosis.
 
Step 2. ASFV Real-time PCR Diagnosis
1. Add 5 μL of the clear supernatant into a 0.2 mL tube containing 17 μL premix of Palm PCR™ ASFV Fast PCR Kit, and mix well.
2. Transfer all 22 μL of the mixed solution into a Type IIA Palm PCR™ sample tube and close with an optical cap.
3. Centrifuge the Palm PCR™ sample tube for 2 min at 8,500 RPM on Ahram™ RX1 Portable Centrifuge, and perform hot-start real-time PCR on Palm PCR™ S1/S1e device.
 


Ultra-Fast MDx with Extreme Sensitivity
Sensitivity of the ASF Fast POCT System
Fast POCT diagnosis of ASF using the Direct Prep method can deliver accurate results in a short turnaround time (within 40 min) with very high sensitivity that is equivalent to typical DNA extraction methods.

Sensitivity of the Direct Prep method is tested using the URL-ASF reference sample (ASFV positive serum) obtained from the European Union Reference Laboratory for ASF (EURL-ASF), CISA-INIA, Spain. The URL-ASF reference sample was serially diluted with an ASFV negative serum and each diluted serum sample was processed with Palm PCR™ ASFV Direct Prep Kit. The processed sample was tested using Palm PCR™ ASF Fast POCT Kit on Palm PCR™ S1/S1e device.

Shown on the right are results of the ASF Fast POCT diagnosis using the Direct Prep method. As shown, the ASF Fast POCT diagnosis successfully detect ASFV DNA at 1/10,000 dilution with 100% hit rate, and at 1/100,000 dilution (about 2.5 copies/reaction) with about 70% hit rate. Serial dilutions with an ASFV negative plasma also delivered identical results (data not shown).
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